3R is an integral part of experimental research in the life sciences. We are committed to advancing the knowledge, the implementation and use of 3R principles as follows.

Key position statement

Education:

• All stakeholders in experimental research must be aware of 3R principles and their current implementation.
• 3R education needs to improve at all levels. This also includes the authorities/deciding bodies.
• 3R is not only relevant to researchers, but also to (strategic) decision takers, i.e. to everybody in all relevant organizations.
• Peer reviewers must be informed better about use, dissemination and quality control of 3R methods.
• We need to promote the awareness that good science includes today’s standards in reporting, 3R standards, etc.

3R related knowledge:

• 3R related issues must be an integral part of scientific publications.
• Methodology with potential impact on the 3R’s must be described in detail. Scientific journals should give sufficient space for description of methodology (e.g. according to ARRIVE-guidelines).
• Papers should include a “3R section” where 3R methods can be described and (more important) new 3R developments that occurred during current research project.
• A comprehensive database related to 3R technology is necessary.

Implementation strategies also requiring external input:

• More research needs to be done in 3R methods, the financial and regulatory support in particular for Refinement has to improve.
• We are committed to implement more 3R methods in our research but this must include additional funding schemes.
• We commit to review and to collaborate in the establishment of release guidelines of established and validated methods.
• We recommend the establishment of key performance indicators for the implementation of 3R principles.